Development and assessment of microsatellites and AFLPs for Plutella xylostella
نویسندگان
چکیده
If we are to both understand and manipulate diamondback moth populations, a thorough grasp of P. xylostella population genetics is essential. However, reliable and informative molecular markers are currently unavailable. We have developed P. xylostella microsatellites and report preliminary results on their variability within and between selected populations. We characterised twelve microsatellite loci with 5–16 alleles within a small geographical area (Cameron Highlands, Malaysia) and up to 57 alleles across populations from nine countries. These loci should prove valuable tools for understanding P. xylostella gene flow and population biology. The AFLP approach also generated numerous variable markers (up to 156 informative bands per restriction enzyme pair) and offers an apparently quicker and cheaper alternative. However, assigning fractions of AFLP variability to different sources suggests that these anonymous markers are less consistent in performance and may sometimes detect loci in P. xylostella symbionts and parasites. The procedures required to remove or control for parasite AFLP reduce the time and cost benefits of AFLPs for population studies and we suggest that microsatellites provide the best immediate prospects for P. xylostella population genetics.
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